How to increase experimental throughput and reproducability

Answer

This article details some best practices to increase experimental throughput

Hints & Tips

Increase experimental throughput by:

• Better arranging the wells/tubes used within SQWERTY so there is less movement required between transfers.

• Use the multi-dispense operation

• Decrease the volumes used per reaction

• optimise liquid parameters that relate to speed

Increase reproducability by:

• Improving your understanding of liquid handling theory and fluid dynamics.

• Use that knowledge to optimise your advanced parameters and liquid classes.

• Use the same high quality labware consistently across all experiments.

• Use GLP and work in an environmentally controlled location

  • Ideally 20 – 25 °C, 30-60% humidity, low air flow.

If you require more information please contact [e-mail] for assistance.