What is Liquid Level Tracking (LLT)?

Answer

As SQWERTY aspirates Liquid Level Tracking (LLT) will cause the tip to gradually move downwards to maintain the depth as constant.

The position the tip is in after the aspiration is then set as the new surface level, so when the pipette tip returns it will immediately return to that position. LLT will then kick in again as the tip aspirates.

Use of Liquid Level Tracking (LLT) requires a greater degree of object shape accuracy because the calculations it performs use the object dimensions to determine the distance the liquid surface would rise/fall according to the volume change. This is especially critical when Liquid Level Detection (LLD) is being used as without LLT there will be air aspiration. However you might just get around this by increasing the LLD Extra travel to compensate for any errors in LLT. This may compromise on volume accuracy from drips adhered to the outside of the tip. But again these are risks for you to weigh up dependent on your application.

Inaccuracies in object files and unoptimised liquid classes/advanced parameters can all effect the effectiveness of LLD and LLT.

If you require more information please contact [e-mail] for assistance.