What is the recommended volume when using SQWERTY?

Answer

When considering the volume required the first question to ask is: How much volume is actually required per reaction?

If you use a lower volume then SQWERTY will be able to finish all the transfers faster using the multi-dispensing aliquot function.

If possible, create a master mix and then use the multi-dispensing workflow. Not only will this mean less reagent is used overall, but the reaction contents will be more consistent between repeats.

On the other hand, low volume pipetting is generally less accurate so may require further optimisation.

What if the user requires one volume that is vastly different from the majority in the experiment?

Although SQWERTY does come with 3 pipette module options, it is not possible to change the pipette module mid-workflow.

• If the volume is too big - use multiple transfers of equal volume. e.g. total transfer of 350 μl would require 2 transfers of 175 μl in the 250 μl pipette module.

• If the volume is too small - dilute the source with PBS or another neutral buffer before transferring the liquid. If this is not possible then the user must accept some slight accuracy errors. The 250 μl pipette module total % error rises to 5.5% when transferring 5 μl into liquid.

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