Can the ROTOR(+) use compounds?

Answer : It is possible to use compounds in the ROTOR. Here are some examples of questions related to using compounds in the ROTOR(+).

Questions :

1. Has anyone used your pads to apply compounds dissolved in water or DMSO to the surface of a yeast agar plate
2. Are there any recommendations for particular solvents, or other parameters?
3. How uniform is the application volume from plate to plate?

Answers : Most chemical screens are done in liquid. Currently, we don't know of any customers who transfer compounds from liquid onto plates. There are a few customers who use the ROTOR(+) to pin liquids onto plates.

Rodney Rothstein's group used the ROTOR for spotting serially diluted cells. (Pubmed ID: 23589890)

Steve Oliver's group used the ROTOR to transfer cells with different ethanol concentrations. (http://linkinghub.elsevier.com/retrieve/pii/S0961953412002498)

In general, liquid transfer is done by capillary action on the surface of the tips, which usually transfers around 100 nl +/- 15%. Rothstein's group has reported that the volume from the first transfer varies quite a bit. However, if the same RePad is used for the subsequent transfer, the volume becomes quite consistent and accurate.

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