How to sample from one source plate to multiple target plates without returning to source (dilution) on the ROTOR(+)?

Answer : This is a simple job to do on the ROTOR(+). It uses the Recycle options in the ROTOR(+) Software. Please see below for full instructions

Please check the first video in this article : A demonstration of the advanced options in the ROTOR Software (Videos). It provides a walk-through of how to access the necessary options. 

A breakdown of the procedure would be (time location in video): 

• Choose pad source and target densities as normal
• Select Replicate Many
• From the General tab choose the number of target plates (1:15)
• Select Recycle tab and select your Recycle option. A recommendation would be 'Until Repeat' (1:45) - this will throw the pad away after pinning all four plates
• Do not select the 'Use?' box - (2:01) - this will allow it to use the same pad for all 4 plates but will not return to the source plate in between each pinning.

If you require more information please contact [e-mail] for assistance.