Random Colony Screening Protocol

Ian -

If you need to pick randomly plated colonies into 96 well plates and screen for the phenotype in liquid culture? It would be beneficial to include a preliminary screening step on agar plates. The strains that pass the preliminary screening step would then be picked into 96-well plate for follow-up screens. Doing a preliminary screen on agar plate could potentially increase your throughput and the quality of your data.

The attached PDF explains the process.

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