Below are examples of some questions we have been asked about using compounds on the ROTOR. There are references to papers which have been produced by 2 of our customers who have been using the ROTOR for a number of years.
- Can you tell me if anyone has used your pads to apply compounds dissolved in water or DMSO to the surface of yeast agar plate
- Are there any recommendations for particular solvents, or other parameters?
- How uniform is the application volume from plate to plate?
Answers : Most chemical screens are done in liquid. Currently we don't know of any customers who transfer compounds from liquid onto plates. There are a few customers who use the ROTOR to pin liquids onto plates however.
Rodney Rothstein's group uses the ROTOR for spotting serially diluted cells. (Pubmed ID: 23589890)
Steve Oliver's group uses the ROTOR to transfer cells with different ethanol concentrations. (http://linkinghub.elsevier.com/retrieve/pii/S0961953412002498)
In general liquid transfer is done by capillary action on the surface of the tips, which usually transfers around 100 nl +/- 15%. Rothstein's group have reported that the volume from the first transfer varies quite a bit. However, if you use the same repad for subsequent transfer, the volume becomes quite consistent and accurate.