How to make Agar

Ian -

This educational video shows you how to make and pour 400ml of YPD Agar.

 Agar must be autoclaved prior to use to avoid growth of contaminants. However there are alternatives: -

  • Gamma Irradiation
  • Electron-beam linear acceleration 
  • Ethylene Oxide Gas 


  • 4g Yeast Extract 
  • 8g Bacterial Peptone 
  • 8g Glucose 
  • 6.4g Agar 
  • 400ml Water 
  • Antibiotic (varies depending on use)

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